p53 Protein, Clone DO-7, Concentrate, 0,2 ml

p53 Protein, Clone DO-7, Concentrate. Specifications:- Antibody Solution: Concentrate. Clone: DO-7. Code No: M7001. EAN/UPC Code: 05700572013327. Form/Configuration: Culture supernatant. Full Product Name: Monoclonal Mouse Anti-Human p53 Protein, Clone DO-7. Immunogen: Recombinant human wild-type p53 protein (1). See package insert for reference(s). Isotype: IgG2b, kappa. JAN Code: 4987582019810. Pathology Application: Frozen;Formalin;HIER. Reagent Provided: Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN 3 . Species: Mouse Anti-Human. Specificity (Detailed): SDS-PAGE analysis of immunoprecipitates formed between lysate of the BT474 breast cancer cell line and the antibody shows reaction with a 53 kDa protein corresponding to p53 (1). In Western blotting of lysate of the A431 human vulval carcinoma cell line, the antibody labels a 53 kDa band, corresponding to the mutant type p53, which is expressed by A431. The epitope recognized by the antibody is located between the N-terminal amino acids 1 and 45 and possibly between amino acids 37 and 45 of the human p53 protein (1). In IHC, the antibody labels mutant-type p53 in the A431 cell line and wild-type p53 in the SVK14 cell line (SV40-transformed keratinocyte line) (1). See package insert for reference(s). Volume: 0.2 mL.