MITF, Clone D5, Concentrate

MITF, Clone D5, Concentrate. Specifications:- Antibody Solution: Concentrate. Clone: D5. Code No: M3621. EAN/UPC Code: 05700571102480. Form/Configuration: Culture supernatant. Full Product Name: Monoclonal Mouse Anti-Human MITF, Clone D5. Immunogen: Histidine fusion protein of the amino-terminal Taq-Sac fragment of human MITF cDNA (1,2). See package insert for reference(s). Isotype: IgG1, kappa. JAN Code: 4987582028102. Pathology Application: Formalin;HIER. Reagent Provided: Monoclonal mouse antibody provided in liquid form as tissue culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2, and 0.015 mol/L sodium azide. Contains stabilizing protein. Species: Mouse Anti-Human. Specificity (Detailed): In Western blot anaylsis of human melanoma cell line, 501mel, anti-MITF demonstrated 54 and 60 kDa bands corresponding to MITF variants differing in phosphorylation status. Further, specificity was demonstrated in gel-mobility assays where anti-MITF produced a specific gel-mobility supershift with MITF but not with related bHLHzip factors TFEB, TFEC and TFE3 (1). See package insert for reference(s). Volume: 0.2 mL.