Epithelial Antigen, Clone Ber-EP4, Concentrate, 1 ml

Epithelial Antigen, Clone Ber-EP4, Concentrate. Specifications:- Antibody Solution: Concentrate. Clone: Ber-EP4. Code No: M0804. EAN/UPC Code: 05700572005629. Form/Configuration: Culture supernatant. Full Product Name: Monoclonal Mouse Anti-Human Epithelial Antigen, Clone Ber-EP4. Immunogen: MCF-7 cells (human breast carcinoma cell line) (4). See package insert for reference(s). Isotype: IgG1, kappa. JAN Code: 4987582007503. Pathology Application: Frozen;Formalin;HIER. Reagent Provided: Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN 3 . Species: Mouse Anti-Human. Specificity (Detailed): Analysis of immunocomplexes between the antibody and lysate of 125 I surface-labeled MCF-7 cells in SDS-PAGE under reducing conditions shows that the antibody labels two polypeptides of 34 kDa and 39 kDa, respectively, corresponding to epithelial antigen. Under non-reducing conditions, the polypeptides appear as 39 kDa and 41 kDa, while deglycosylation reduces the size to 31 kDa, and 36 kDa. In immunoprecipitation experiments, the Ber-EP4 antibody blocks the reaction of the HEA125 antibody with MCF-7 cell lysate and vice versa, showing that the two antibodies react with the same antigen. The two antibodies also produce identical staining results in cells and tissues (4). Of 37 cell lines tested, the antibody homogeneously labels all (10/10) carcinoma cell lines, whereas all non-epithelial cell lines (26/27) are not labeled except for the erythromyeloid cell line K562 (4). See package insert for reference(s). Volume: 1 mL.