CD79 Alpha , Clone JCB117, Concentrate

CD79 Alpha , Clone JCB117, Concentrate. Specifications:- Antibody Solution: Concentrate. Clone: JCB117. Code No: M7050. EAN/UPC Code: 05700572006329. Form/Configuration: Culture supernatant. Full Product Name: Monoclonal Mouse Anti-Human CD79 Alpha , Clone JCB117. Immunogen: Recombinant protein containing part of the extracellular portion of the human CD79 Alpha glycoprotein (1). See package insert for reference(s). Isotype: IgG1, kappa. JAN Code: 4987582008555. Pathology Application: Formalin;HIER. Reagent Provided: Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN 3 . Species: Mouse Anti-Human. Specificity (Detailed): The antibody was clustered as anti-CD79 Alpha at the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens held in Kobe in 1996 (4). In Western blotting of Ramos B-cell lysate, the antibody labels a band corresponding to CD79 Alpha under reducing conditions, and a band corresponding to CD79 Alpha B under non-reducing conditions (1). SDS-PAGE analysis of immunoprecipitates formed between the antibody and 125 I-labeled, mildly denatured, and reduced lysate of Ramos B cells, shows weak reaction with a 43 kDa polypeptide corresponding to CD79 Alpha . The antibody was tested by flow cytometry against normal peripheral B-cell lines, and against mature and immature B-cell lines (Ramos, Raji, and NALM-1), and was consistently negative, indicating that the epitope recognized is not present, or is not accessible, on living cells. However, the antibody specifically labels B cells in paraffin-embedded tissue sections (4). See package insert for reference(s). Volume: 1 mL.