CD3, Concentrate, 1 ml

CD3, Concentrate. Specifications:- Antibody Solution: Concentrate. Clone: Polyclonal. Code No: A0452. EAN/UPC Code: 05700572001461. Form/Configuration: Affinity isolated. Full Product Name: Polyclonal Rabbit Anti-Human CD3. Immunogen: Synthetic peptide comprising amino acids 156-168 from the cytoplasmic part of the human CD3e-chain coupled to thyroglobulin (1). See package insert for reference(s). JAN Code: 05700572001461. Pathology Application: Frozen;Formalin;HIER. Reagent Provided: Affinity-isolated rabbit anti-human CD3 provided in liquid form. In 0.05 mol/L Tris-HCl, 0.1 mol/L NaCl, 15 mmol/L NaN 3 . The affinity isolation has been performed using immobilized CD3 peptide. Species: Rabbit Anti-Human. Specificity (Detailed): In Western blotting, the antibody detects bands of the expected molecular weights for CD3 antigens (2). The antibody recognizes CD3E in both a T-cell line (Jurkat) and a natural killer cell line (NK11), but does not react with lysates prepared from several B-cell lines (Raji, Ramos and JY), a myeloid cell line (U937) or a colon carcinoma cell line (Colo-205) (9).In immunoprecipitation from Nonidet P40 lysates of surface-iodated T lymphoblasts, the antibody precipitates the Gamma (26 kDa), Delta (21 kDa) and E (19 kDa) chain of the CD3 molecule, similar to the precipitation pattern seen using the well-characterized monoclonal mouse anti-human CD3, clone UCHT1 (1).In ELISA, the antibody labels the CD3 peptide used as immunogen. See package insert for reference(s). Volume: 1 mL.