CD117, c-kit, Concentrate

CD117, c-kit, Concentrate. Specifications:- Antibody Solution: Concentrate. Clone: Polyclonal. Code No: A4502. EAN/UPC Code: 05700571101582. Form/Configuration: Affinity isolated. Full Product Name: Polyclonal Rabbit Anti-Human CD117, c-kit. Immunogen: Peptide corresponding to amino acids 963 to 976 at the cytoplasmic C-terminal part of c-kit (1,5). See package insert for reference(s). JAN Code: 4987582025620. Pathology Application: Formalin;HIER. Reagent Provided: Affinity-isolated rabbit antibody purified through antigen-bound, activated thiol AvidGel F affinity chromatography and provided in liquid form, in 0.05 mol/L Tris-HCl, 0.1 mol/L NaCl, 0.015 mol/L NaN 3 , pH 7.2, 1% bovine serum albumin. Species: Rabbit Anti-Human. Specificity (Detailed): In Western blotting of an extract of the small cell lung carcinoma cell line SY, that over-expresses c-kit mRNA, the antibody labels a band of 145 kDa corresponding to the c-kit protein. The labeled band is rather broad, from 120 to 155 kDa. An additional band of 100 kDa is also labeled (1). In another study applying a different antibody to c-kit, a 100 kDa protein was, likewise, labeled. This labeling was abolished when the antibody was incubated with the synthetic c-kit peptide antigen used for immunization (6). In Western blotting, the antibody did not react with an extract of the adenocarcinoma cell line HS, that is without c- kit gene expression (1). See package insert for reference(s). Volume: 0.2 mL.